Addgene: Luciferase-pcDNA3 Sequences

Full Sequences from Depositor 1 Sequence provided by depositing laboratory may be theoretical/predicted or based on Sanger/NGS sequencing results, Discrepancies between sequencing results obtained by Addgene and the original sequence provided by the depositor may be present, Analyze Sequence,

Firefly luciferase gene: structure and expression in

Although the luciferase cDNA clone lacked the six N-terminal codons of the open reading frame, we were able to reconstruct the equivalent of a full-length cDNA using the genomic clone as a source of the missing 5′ sequence, The full-length, intronless luciferase gene was inserted into mammalian expression vectors and introduced into monkey CV-1 cells in which enzymatically active firefly

Analysis of Regulatory DNA Sequences by Dual-Luciferase

Here, we describe the process of analyzing the enhancer activity of regulatory DNA sequences using transfection-based dual-luciferase reporter assays in Ewing sarcoma cell lines, To this end, we provide a protocol for cloning sequences of interest from genomic DNA into a firefly luciferase-containing plasmid, transfecting Ewing sarcoma cells with plasmids and measuring luciferase expression by

Firefly luciferase gene: structure and expression in

The nucleotide sequence of the luciferase gene from the firefly Photinus pyralis was determined from the analysis of cDNA and genomic clones, The gene contains six introns, all less than 60 bases in length, The 5′ end of the luciferase mRNA was determined by both S1 nuclease analysis and primer extension, Although the luciferase cDNA clone lacked the six N-terminal codons of the open reading

Real-time DNA sequencing using detection of pyrophosphate

The PPi formed in the DNA polymerase reaction is converted to ATP by ATP sulfurylase and the ATP production is continuously monitored by the firefly luciferase, In the sequencing procedure, immobilized single-stranded template was used in a repeated cycle of deoxynucleotide extension, Real-time signals in the ELIDA, proportional to the amount of incorporated nucleotide, were observed when

The Luciferase Reporter Assay: How it Works & Why You

Date de publication : sept, 22, 2021Temps de Lecture Estimé: 3 mins

The luciferase reporter assay is commonly used as a tool to study gene expression at the transcriptional level, It is widely used because it is convenient, relatively inexpensive, and gives quantitative measurements instantaneously, It has broad applications across various fields of cell and molecular biology—wherever you want to measure or

The Reaction at The Heart of The Luciferase Reporter AssayLuciferases make up a class of oxidative enzymes found in several species that enable the organisms that express them to “bioluminesce,” or emit liCarrying Out The Luciferase Reporter Assay ItselfThere are several commercially available luciferase reporter assay kits comprising expression vectors that contain the luciferase reporter gene orWhy You Should Use The Luciferase Reporter Assay…This reporter assay can be used to study gene expression as well as other cellular components and events that are involved in gene regulation, Its

In vitro transcription and capping of Gaussia luciferase

Linearized plasmid DNA, PCR products and synthetic DNA oligonucleotides can be used as templates for transcription as long as they have the T7 promoter sequence upstream of the gene to be transcribed, Addition of a 5′ end cap structure to the RNA is an important process in eukaryotes, It is essential for RNA stability8, efficient translation9, nuclear transport10 and splicing11, The

pGL3 Luciferase Reporter Vectors

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Information on related products, including the Luciferase Assay System, is provided in Sections 4–7 and 8,K, Storage Conditions: Store the pGL3 Luciferase Reporter Vectors at –20°C, 3, pGL3 Vector Maps and Sequence Reference Points The listings of restriction sites for the pGL3 Luciferase Reporter Vectors are provided in Section 8,G–J,

Bases de la résistance du VIH aux ARV

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DNA DNA DNA Cibles des antirétroviraux Inhibiteurs d’entrée Inhibiteurs de la TI Inhibiteurs de Protéase Inhibiteurs du bourgeonnement Inhibiteurs de l’intégrase Taux d’erreur de la TI : 1/10,000 nucléotides Production virale : 109-1010 particules par jour Taux de recombinaison : 5 à 10 évènements par cycle Evolution constante de la quasiespèce Toutes les mutations pré-existent

Les techniques de séquençage de l’ADN : une révolution en

DNA sequencing is an essential tool in molecular biology and applied biosciences, Described in the late 1970s, this method had enormously increased the possibilities of genetic research, DNA sequencing is now routinely used in molecular biology laboratories, This technology has allowed sequencing of various and important genomes as the human one, Numerous innovations to improve DNA sequencing

Adeno-Associated Virus Serotype-Specific Inverted Terminal

Colony plasmids were screened for intact ITR sequences by using restriction enzymes specific to the ITR genotype and then cloned into a pUC19 backbone with a 20 nt stuffer sequence chosen randomly from lambda phage DNA each plasmid contained the same stuffer sequence, followed by the reading frame for luciferase or cre recombinase, an SV40 early polyA signal, and …

Isolation and expression of a cDNA encoding Renilla

The recombinant luciferase expressed in Escherichia coli is identical to native luciferase as determined by SDS/PAGE, immunoblot analysis, and bioluminescence emission characteristics, Full text, Full text is available as a scanned copy of the original print version, Get a printable copy PDF file of the complete article 1,0M, or click on a page image below to browse page by page, Links to

NanoLuc: A Small Luciferase is Brightening up the Field of

Luciferase is a general name for enzymes that produce light in living organisms, There are two key requirements for the production of bioluminescence, including the enzyme responsible for catalyzing the reaction and producing light luciferase and the substrate for this enzyme luciferin, Many such systems have been characterized, with several famous examples outlined in Figure 2, For an

Transient and Stable Expression of the Firefly Luciferase

The luciferase gene from the firefly, Photinus pyralis, was used as a reporter of gene expression by light production in transfected plant cells and transgenic plants,A complementary DNA clone of the firefly luciferase gene under the control of a plant virus promoter cauliflower mosaic virus 35S RNA promoter was introduced into plant protoplast cells Daucus carota by electroporation and

Using Luciferase to Image Bacterial Infections in Mice

Luciférase est génétiquement introduits dans les bactéries pour Définissez les paramètres pour l’imagerie en cliquant dans le panneau de configuration séquence IVIS de contrôle d’acquisition, Sélectionnez la luminescence et de photographier en mode imagerie, Si l’option d’effectuer DLit reconstruction 3D est souhaitée, sélectionnez photographique, luminescentes et structurelles