Double Digests

Setting up a Double Digestion Double digests with NEB’s restriction enzymes can be set up in rCutSmart Buffer™, Otherwise, choose an NEBuffer that Set up reaction according to recommended protocol, The final concentration of glycerol in any reaction should be less If two different incubation

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Double Digests

Setting up a Double Digestion Double digests with NEB’s restriction enzymes can be set up in rCutSmart Buffer™, Otherwise, choose an NEBuffer that Set up reaction according to recommended protocol, The final concentration of glycerol in any reaction should be less If two different incubation

Double Digest Protocol with Standard Restriction Enzymes

Also, NEB‘s online tool NEBcloner ® will help guide your reaction buffer selection when setting up double digests, Setting up a Double Digestion In most cases, double digests with NEB‘s restriction enzymes can be set up in rCutSmart Buffer, Otherwise, choose an NEBuffer that results in the most activity for both enzymes, If star activity is a concern, consider using one of our High Fidelity

Double Digest Protocol with Standard Restriction Enzymes

Also, NEB‘s online tool NEBcloner ® will help guide your reaction buffer selection when setting up double digests, Setting up a Double Digestion In most cases, double digests with NEB‘s restriction enzymes can be set up in rCutSmart Buffer, Otherwise, choose an NEBuffer that results in the most activity for both enzymes, If star activity is a concern, consider using one of our High Fidelity

Restriction Digest Protocol

Restriction Digest Protocol, A specific protocol for single digestion using this restriction enzyme can be accessed using our free online tool, NEBcloner , Please note that NEBcloner will also provide detailed double digest protocols using this enzyme, Additional information on performing digests using restriction enzymes can be found in our

Double Digest Protocol using One RE-Mix and One Standard

Double Digest Protocol using One RE-Mix and One Standard Restriction Enzyme Protocol, Dilute up to 1 μg DNA to 17 μl with dH 2 O ; Add 2 μl of the 10X RE-Mix and 1 μl of the standard enzyme ; Incubate at 37°C, for 15 minutes Time-Saver enzymes, or 1 hour for standard enzymes; Analyze by agarose gel electrophoresis; Note: Use only with standard restriction enzymes with 37°C …

Restriction Digest Protocol

Restriction Digest Protocol A specific protocol for single digestion using this restriction enzyme can be accessed using our free online tool, NEBcloner,Please note that NEBcloner will also provide detailed double digest protocols using this enzyme,

NEBcloner

Traditional Cloning Workflow, Restriction Digest End Modification Ligation Transformation, Site Directed Mutagenesis SDM Transformation, Select a workflow step below to …

CutSmart® Buffer

Over 215 restriction enzymes are 100% active in CutSmart Buffer, making it significantly easier to set up your double digest reactions, Since CutSmart Buffer includes BSA, there are also fewer tubes and pipetting steps to worry about, Additionally, many

Optimizing Restriction Endonuclease Reactions

Enzyme

NEB Restriction Enzyme Double Digest Protocol

Double digestions can save you time, and this video can offer tips for how to achieve the best results, Learn more at https://www,neb,com/applications/clonin

CutSmart® Buffer

CutSmart, Buffer, This product will be discontinued on 12/15/2021 and is replaced with rCutSmart Buffer NEB #B6004, Over 215 enzymes are 100% active in CutSmart ® Buffer simplifying double digests, Visit NEBCutSmart,com to learn more about the CutSmart Buffer system, Featured Video,

Addgene: Molecular Biology Protocol

*Pro-Tip* If you are conducting a double digest digesting with two enzymes at the same time, you will need to determine the best buffer that works for both of your enzymes, Most companies will have a compatibility chart, such as the double digest finder tool from NEB,