Gateway pENTR Vectors

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pENTR™1A Vector 11813-011 pENTR™2B Vector 11816-014 pENTR™3C Vector 11817-012 pENTR™4 Vector 11818-010 pENTR™11 Vector 11819-018 Shipping and Storage pENTR™ vectors are shipped at room temperature, Upon receipt, store at -20°C, Products are guaranteed for six months from date of shipment when stored properly,

pENTR D-TOPO Sequence and Map

pENTR™/D-TOPO® Directional TOPO® cloning vector, for creating a Gateway® entry vector that is suitable for recombination with a destination vector,

Addgene: Vector Database

Welcome to Vector Database!, Vector database is a digital collection of vector backbones assembled from publications and commercially available sources, This is a free resource for the scientific community that is compiled by Addgene,, This page is informational only – this vector is NOT available from Addgene – please contact the manufacturer for further details,

Gateway™ pENTR™ 2B Dual Selection Vector

New: pENTR™ Dual Selection vectors! The Gateway® entry vectors Table 1 offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression, • Kozak consensus sequence for efficient

pENTR1A Sequence and Map

pENTR™1A Gateway® Dual Selection Vector for creating an entry vector by restriction cloning, Other reading frames are available with pENTR™2B and pENTR™3C,

pENTER, P100001

AAV vector with CAG promoter, Read More > pAV-CaMKIIa, AAV vector with CaMKIIa promoter, Read More > Customer Support 301-251-6638 Toll Free: 1-800-485-5808, Ready to get started? Request a Quote, Viral Packaging Services, AAV Packaging; Adenovirus Packaging; Lentivirus Packaging

pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10

The pENTR™/D-TOPO® Cloning Kits utilize a highly efficient, 5-minute cloning strategy “TOPO® Cloning” to directionally clone a blunt-end PCR product into a vector for entry into the Gateway® System or the MultiSite Gateway® System, Blunt-end PCR products clone directionally at greater than 90% efficiency, with no ligase, post-PCR procedures, or restriction enzymes required,

pENTR™ Directional TOPO® Cloning Kits

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pENTR™ Directional TOPO® Cloning Kits Five-minute, directional TOPO® Cloning of blunt-end PCR products into an entry vector for the Gateway® System Catalog numbers K2400-20, K2420-20, K2525-20, K2535-20, K2435-20, and K2635-20 Revision Date …

pET Expression Vectors

prepared vector for ligation-independent cloning, with amino-terminal His•Tag™ sequence : pET-46 Ek/LIC: with HRV 3C Protease cleavage site for efficient fusion tag removal: pET-47b+, pET-48b+, pET-49b+, pET-50b+ Nus•Tag™ fusion increases the solubility of recombinant annexin A1, pENTR™ vectors containing the annexin A1 target gene were generated using the Gateway Nova pET-53

Addgene: pENTR-AtMIR390a-B/c

Plasmid pENTR-AtMIR390a-B/c from Dr, James Carrington’s lab contains the insert AtMIR390a-B/c and is published in Plant Physiol, 2014 Mar 19, This plasmid is available through Addgene,

pENTR™ Directional TOPO® Cloning Kits

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pENTR™ TOPO® vector, TOPO ®-adapted pENTR™/D-TOPO® or pENTR™/SD/D-TOPO® or pENTR™/TEV/D-TOPO® 15-20 ng/µl linearized plasmid DNA in: 50% glycerol 50 mM Tris-HCl, pH 7,4 at 25°C 1 mM EDTA 2 mM DTT 0,1% Triton X-100 100 µg/ml BSA 30 µM bromophenol blue 20 µl dNTP Mix 12,5 mM dATP 12,5 mM dCTP 12,5 mM dGTP 12,5 mM dTTP in water, pH 8 10 µl Salt Solution …

Vector Backbone pENTR223-1

pENTR223-1 vector backbone, + Sequence information, + Datasheet, + Compare & Order pENTR223-1 vector backbone products + TOP customer support,

Pentr U6 Vector

pentr u6 vector, , Thermo Fisher, The BLOCK iT U6 RNAi Entry Vector provides a simple streamlined approach to cloning short hairpin RNA shRNA sequences for testing in transient transfections for RNA interference RNAi a preferred technique for analyzing gene downregulation An easy cloning process places an 50 bp oligonucleotide DNA

Gateway pENTR Dual Selection Vectors

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pENTR™3C Dual Selection Vector A10464 pENTR™4 Dual Selection Vector A10465 pENTR™11 Dual Selection Vector A10467 Shipping and Storage pENTR™ Dual Selection Vectors are supplied in TE buffer and shipped on wet ice, Upon receipt, store at -20°C, Products are guaranteed for six months from date of shipment when properly stored, Contents

Protocol: Streamline cloning of genes into binary vectors

A, Creating the Entry clone using pENTR™ Directional TOPO ® cloning kit, Using pENTR/D-TOPO ® cloning kit, the genes, or DNA fragments of interest, are PCR- amplified and readily introduced into pENTR™ D-TOPO ® cloning vector to generate the Entry clone, The key steps are as follows: 1, Design PCR primers for gene amplification, with an addition of 4-nucleotide CACC at the 5′ …